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. 2015 Jan 20;116(3):476–485. doi: 10.1002/jcb.25001

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© 2014 The Authors. Journal of Cellular Biochemistry published by Wiley Periodicals, Inc.

This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.

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MBD4 complexes with UHRF1 and USP7. (A) UHRF1 and USP7 are identified as new interaction partners of MBD4. MBD4 was immunoprecipitated from nuclear extracts with MNase treatment and subjected to SDS–PAGE and Coomassie blue staining. Protein bands of UHRF1 and USP7 specific for the FLAG‐MBD4‐IP were identified by MS analysis (ctrl: Empty vector). Precipitated MBD4 and the molecular weight marker (M) are indicated. (B) Immunoprecipitation of ectopic proteins with FLAG epitope‐tagged (MNase treated nuclear extract) or GFP epitope‐tagged (no MNase treated nuclear extract) with anti‐FLAG or anti‐GFP antibodies. Co‐precipitated proteins were detected by Western Blot analysis. 3% of the input (Lane 1) and the antibody coupled IP (Lane 2) were loaded. The migration of the molecular weight is indicated on the left.