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(a) To investigate cell fate at low flow rates, each cell ejection was followed by needle washes (3 × 100 μl of phosphate buffered saline (PBS) ejected at 300 μl/min each), and the number of cells recovered was measured using PrestoBlue (mean ± SEM, n = 3). (b) Ejections through needles of various gauges and lengths were followed by washes using 3 × 100 μl of PBS ejected at 300 μl/min each, and the number of cells recovered was measured using PrestoBlue (mean ± SEM).
