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. 2016 Feb 26;3(2):328–339. doi: 10.1080/23328940.2016.1153360

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© 2016 The Author(s). Published with license by Taylor & Francis Group, LLC

This is an Open Access article distributed under the terms of the Creative Commons Attribution-Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. The moral rights of the named author(s) have been asserted.

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Figure 2. — Immunoblot showing expression of RyR1 constructs Immunoblot of HEK293T cells transiently transfected with RYR1 expression plasmids. Total protein (270 µg) extracts were separated on a 7.5% SDS-PAGE gel and transferred onto a PVDF membrane. RyR1, > 250 kDa, and α-tubulin, 50 kDa, were detected using anti-RyR1 (34C) and anti-α-tubulin antibodies respectively. Lane 1, pcDNA3.1+ vector only; lane 2, pcRyR1 wildtype; lane 3, pcRyR1 Gly248Arg; lane 4, pcRyR1 Arg2452Trp; lane 5, pcRyR1 Thr214Met; lane 6, pcRyR1 Thr214Met/ΔGlu2348; lane 7, pcRyR1 ΔGlu2348.