Table 2.
EC50 values 4-CmC.
| construct | EC50 µM 4-CmC | SEM | p-value |
|---|---|---|---|
| WT | 481 | 15.00 | |
| Gly248Arg | 270 | 6.00 | *8.21×10−4 |
| Arg2452Trp | 341 | 7.70 | *6.6×10−4 |
| Thr214Met | 403 | 15.00 | 0.250 |
| Thr214Met/ΔGlu2348 | 219 | 15.22 | *2.08×10−5 |
| ΔGlu2348 | 261 | 20.70 | *1.45×10−4 |
The concentration of 4-CmC required to half maximally activate RyR1 from individual assays was determined using OriginLab Origin 8 software. Results are represented as mean ± SEM. The Student's unpaired T test, followed by a Bonferroni correction, was used to determine the statistical difference between the EC50 value for cells transfected with WT RYR1 cDNA and each variant.