Table 3.

Resting cytosolic Ca²⁺.

construct	Ratio 340/380	SEM	p-value
pcDNA3.1+ vector only	1.030348	0.006029
WT	1.038354	0.002792	0.22291
Gly248Arg	1.030568	0.004414	0.13118
Arg2452Trp	1.061786	0.005017	*2.99 × 10−5
Thr214Met	1.038319	0.003382	0.99381
Thr214Met/ΔGlu2348	1.049861	0.005694	0.05691
ΔGlu2348	1.088946	0.004969	*7.98 × 10−16

Ratio of fluorescence emission at 510 nm using excitation at 340 nm and 380 nm prior to the exposure of RyR1 agonists. Represented is the mean fluorescence emission ratio ± SEM for each construct. The Student's unpaired T test, followed by a Bonferroni correction, was used to determine the statistical difference between cells transfected with the pcDNA3.1+ vector only, and each RyR1 construct.