Table 4.
Oligonucleotide primers.
| Primer name | Sequence | Purpose |
|---|---|---|
| Thr214Met F_SDM | GAAGAGGGCTTCGTGATGGGAGGTCACGTCCTC | Site-directed mutagenesis |
| Thr214Met R_SDM | GAGGACGTGACCTCCCATCACGAAGCCCTCTTC | Site-directed mutagenesis |
| ΔGlu2348 F_SDM | GTCAACGGCGAGAGCGTGGAGAACGCCAATGTGGTGGTGCGG | Site-directed mutagenesis |
| ΔGlu2348 R_SDM | CCGCACCACCACATTGGCGTTCTCCTCCACGCTCTCGCCGTTGAC | Site-directed mutagenesis |
| Thr214Met F_HRM | CAACTTCCCTTGCTCCT | HRM |
| Thr214Met R_HRM | CGCTGGTCATCACTGTCA | HRM |
| ΔGlu2348 F_HRM | CCAGGCGAGAGCGTGGAG | HRM |
| ΔGlu2348 R_HRM | CGGATGAGCAGCCGCACC | HRM |