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. 2016 Jul 28;11(7):e0160103. doi: 10.1371/journal.pone.0160103

Fig 3. Replication history and SHM levels in IGHV genes of natural effector B cells.

Fig 3

A, Replication history of naïve and natural effector B cells as assessed using the KREC assay [25]. B, IGHV mutation frequencies in rearranged IGH genes of natural effector B cells in patients and controls (total numbers of sequences indicated between brackets). Grey dots represent unique sequences; red lines represent median values. C, Selection for replacement mutation in IGHV-CDR (red line) and IGHV-FR regions (blue lines) as determined with the BASELINe program [28, 29]. Solid lines represent patients; dashed lines represent healthy controls. Selection Strengths >0 indicate positive selection. D, IGH-CDR3 size distributions. All individual sizes are indicated as grey dots, red lines representing median values. The dashed line represents median values for centroblasts and centrocytes. Sorted cells were analyzed from patients 15, 16, 18 and 19. Controls were published previously [30, 31]. Statistical analysis was performed with the Mann-Whitney test; *, P<0.05; **, P<0.01; ***, P<0.001; ****, P<0.0001.