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. 2016 Jul 28;11(7):e0159473. doi: 10.1371/journal.pone.0159473

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© 2016 Schmid et al

This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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Fig 8 — A. Hemocytes expressing UAS- ird1^IR with Cg-GAL4 (Cg>ird1^IR), driver control hemocytes (Cg>) and hemocytes extracted from ird1^Δvps15 heterozygote (ird1^-/+) or ird1^Δvps15/ird1⁵ null (ird1^-/-) larvae. Ex vivo hemocytes spread on glass slides were stained with DAPI nuclear dye (blue), AlexaFluor 594- conjugated F-actin-phalloidin (red) and FITC-conjugated α-tubulin antibody (green). B. Total area and C. protrusion area of hemocytes with the same genotypes as in A. Protrusion area is calculated by subtracting the α-tubulin stained region from the total surface spread area stained with phalloidin for each cell using the ImageJ software. Box-plots in B and C depict medians, quartiles and outliers with the number of analyzed blood cells for each genotype indicated in B. P values for pairwise comparisons using Kruskal-Wallis ANOVA are indicated above horizontal bars in B and C. Blood cell images are maximum intensity projections of z-stacks.