Fig 3. Lack of Elovl6 altered fatty acid composition in costal primary chondrocytes.
(A) Lack of Elovl6 significantly increases C16:0 and decreases C18:1 (n-9) in primary chondrocytes. Gas chromatography analysis was performed to examine fatty acid composition of primary chondrocytes harvested from control and Elovl6-/- mice (n = 3 in each group). (B) Lack of Elovl6 significantly decreases Scd-2 mRNA levels in primary chondrocyte. Quantitative determination of levels of Scd-1 and Scd-2 mRNA was examined by real-time qPCR (n = 4 in each group). *p < 0.05, **p < 0.01, ***p < 0.005 vs. controls.