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. 2016 Jul 28;11(7):e0159930. doi: 10.1371/journal.pone.0159930

Fig 2. Phenotype and genotype of littermates from EphA4f/f and Tie2::Cre breeding paradigm.

Fig 2

(A—F) Reporter labeling of Tie2::Cre is specific to blood vessels. Panel F further confirms eGFP expression specifically to blood vessels. (G) Genotyping using PCR shows a flox primers of about 290bp and a bottom Cre band. Lane 1 and 2: EphA4f/f/Tie2::Cre (KO); Lane 3 and 4: EphA4f/f (WT). Vessel painting of surface pial arterioles of WT (H) show increased numbers in KO (I) mice. Panel J and K are WT and KO endothelial cells cultured from brain endothelial cell progenitor cells. ECs are labeled with CD31 (green) and DAPI (blue). (L) ECs from KO mice do not express EphA4 RNA transcripts.