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. 2016 Jul 28;11(7):e0159092. doi: 10.1371/journal.pone.0159092

Fig 3. miR-203 down regulates GAS41 expression in glioblastoma cell.

Fig 3

(A) Real time PCR studies showing the expression of GAS41 in HNGC2 and U87 cells transfected with miR-203. GAPDH is used as loading control in the experiment.Transfection of both the cell lines with empty vector (EV) served as control. Bars represent relative GAS41 mRNA expression normalized to GAPDH from same sample. ** represents p value<0.01 vs empty vector transfected control. (B) Western blots showing expression of GAS41 protein in HNGC2 and U87 cells transfected with miR-203 mimics. (C) Immunofluorescence studies showing the expression and localization of GAS41 in HNGC2 and U87 cells transfected with miR-203. Scale bars represent 5μm. (D) Quantitative RT-PCR studies showing expression of miR-203 in HNGC2 and U87 cells transfected with antimiR-203. *** represents p value <0.001, ** represents p value <0.01vs control. (E) Quantitative RT-PCR studies showing expression of GAS41 after exposure to anti-miR-203 in HNGC2 and U87 cells. ** represents p value <0.01vs control (F) Quantitative Real time PCR studies showing expression of miR-203 in HNGC2 and U87 cells upon over expression of GAS41.** represents p value <0.01 and *** represents p value <0.001 vs EV (cells transfected with pCMVTag1 vector). All the above experiments were performed in triplicates.