Fig 2. Effect of antibiotic treatment on the permeabilization of C. glutamicum expressing DAEase from F. plautii for the production of d-allulose from d-fructose.

(A) Effect of antibiotic treatment. 0 (white bar with diagonal line), 2 (black bar), 5 (white bar), 50 (gray bar), and 100 mg/L (gray bar with diagonal line). (B) Effect of penicillin concentration. The reactions were performed in 50 mM PIPES buffer (pH 7.0) containing 7.5 g/L cells and 50 mM d-fructose at 65°C for 10 min. Data represent the means of three separate experiments and error bars represent the standard deviation.