. 2016 Jul 28;11(7):e0160044. doi: 10.1371/journal.pone.0160044

Table 1. Biochemical properties of DAEases and DTEases.

Enzyme	pH	Temp. (°C)	Metal ions	Half-life at 60°C (min)	k_cat (1/s)		K_m (mM)		k_cat/K _m (1/mM/min)		d-Allulose (g/L)[d-Fructose, g/L]	Reference
Enzyme	pH	Temp. (°C)	Metal ions	Half-life at 60°C (min)	d-Allulose	d-Fructose	d-Allulose	d-Fructose	d-Allulose	d-Fructose	d-Allulose (g/L)[d-Fructose, g/L]	Reference
A. tumefaciens DAEase	8.0	50	Mn²⁺	4^b	40	35	12	24	205	85	230 [700]	[9]
Clostridium sp. DAEase	8.0	65	Co²⁺	10^c	537	273	228	279	141	59	120 [500]	[10]
C. bolteae DAEase	7.0	55	Co²⁺	45^b	49	59	27	60	107	59	216 [750]	[11]
C. cellulolyticum DAEase	8.0	55	Co²⁺	408^c	54	56	17	54	186	63	218 [750]	[12]
C. scindens DAEase	7.5	55	Mn²⁺	50^b	31	9	28	40	65	9	NR	[13]
Desmospora sp. DAEase	7.5	60	Co²⁺	NR^a	86	1,060	81	549	327	116	143 [500]	[14]
Dorea sp. DAEase	6.0	70	Co²⁺	60^c, 30^b	1,311	508	191	153	412	199	115 [500]	[15]
P. cichorii DTEase	7.5	60	None	NR	NR	NR	NR	NR	NR	NR	150 [780]	[7]
R. sphaeroides DTEase	9.0	40	Mn²⁺	NR	NR	NR	NR	NR	NR	NR	118 [700]	[8]
Ruminococcus sp. DAEase	7.5–8.0	60	Mn²⁺	96^b	41	59	48	216	51	16	125 [500]	[16]
T. primita DAEase	8.0	70	Co²⁺	< 30^b	503	292	209	279	144	63	138 [500]	[17]
F. plautii DAEase	7.0	65	Co²⁺	130^b	421	342	162	323	156	64	239 [750]	This study

^aNR, not reported.

^b and ^c represent that the half-life of each enzyme was measured without and with metal ions, respectively.