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. 2016 Jul 28;11(7):e0160108. doi: 10.1371/journal.pone.0160108

Fig 2. Effect of CD13 ligation on neutrophil and recombinant human CD13 activity.

Fig 2

Neutrophils were pre-incubated with bestatin (0.1 mM), anti-CD13 mAb WM-15 (8 μg/ml), anti-CD13 mAb MY7 (5 μg/ml), anti-CD13 mAb WM-47 (1:40 v/v) or vehicle control for 30 min. Cleavage of L-Alanine 4-nitroanilide (2 mM) was measured over 60 min and is expressed as a percentage of the activity observed with control treated cells. Data represent the mean ± SEM of n = 7 (Bestatin), n = 3 (WM-15 and WM-47) or mean ± SD of n = 2 independent experiments (MY7) each performed in triplicate (A). rCD13 (200 ng/ml) was pre-incubated alone or with increasing concentrations of bestatin for 30 min. Cleavage of L-Alanine 4-nitroanilide was measured over 60 min and converted to nmol substrate/min. The mean ± SEM from three independent experiments, each performed in triplicate, are shown. (B). ***, P < 0.001, **, P < 0.01, ns, non-significant vs. control, by Student’s paired t test.