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. 2016 Jul 28;11(7):e0160108. doi: 10.1371/journal.pone.0160108

Fig 3. Effect of CD13 ligation on neutrophil shape change and migration.

Fig 3

Neutrophils were pre-incubated with bestatin (0.1 mM), WM-15 or IgG1 (8 μg/ml), WM-47 (1:40 v/v), MY7 (5 μg/ml) or vehicle control for 30 min (A, B, C and D) or 10 min (E and F). Cells were stimulated in triplicate with a concentration range of fMLP or IL-8 for 10 min and percentage shape change assessed by flow cytometry (A and B). Chemotaxis was assessed in duplicate using a ChemoTx® plate and migration towards a concentration range of IL-8 quantified after 90 min by manual counting (C and D). Migration through collagen I, gelled in a Transwell® insert, towards IL-8 (100 ng/ml) was quantified in duplicate after 9 hr and is expressed as a percentage of the migration observed with control treated cells (E and F). Data represent the mean ± SEM of n = 3 independent experiments (A, C, D, E and F) or mean ± SD of n = 2 independent experiments (B). ***, P < 0.001, *, P < 0.05, ns, non-significant vs. control, by repeated measures ANOVA/Tukey’s test.