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. 2016 Jul 28;11(7):e0160108. doi: 10.1371/journal.pone.0160108

Fig 7. Anti-CD13 mAb WM-15 reduces the proportion of neutrophils entering collagen I.

Fig 7

Collagen I gels were equilibrated over 24 hr with IL-8 (100 ng/ml) or media alone (control). Neutrophils were pre-incubated with bestatin (0.1 mM), WM-15 (8 μg/ml), WM-47 (1:40 v/v) or vehicle control for 10 min and the mean distance migrated and percentage cells entering the gel assessed with live cell imaging. Data represent the mean ± SEM of n = 3 independent experiments. ns, non-significant vs. IL-8 control, by two-way ANOVA/Bonferroni test (A). *, P < 0.05, ns, non-significant vs. 30 min time point, by Student’s paired t test (B). Representative gel surface images at 60 min showing WM-15-induced neutrophil HA. Original magnification x 200 (C and D). WM-15 treated neutrophils at 60 min separated according to whether HA was present or not (E and F). ns, non-significant, by Student’s paired t test.