Skip to main content
. 2016 Jul 22;11:3331–3346. doi: 10.2147/IJN.S106912

Figure 5.

Figure 5

NiONPs induce caspase-1-dependent cytokine secretion in RAW264.7 cells.

Notes: (A–C) The pro-caspase-1 and pro-IL-1β levels in cell lysates (input) and processed caspase-1 and IL-1β levels in culture SNs after treatment with different doses (0 µg/cm2, 1 µg/cm2, 2 µg/cm2, and 4 µg/cm2) of NiONPs were analyzed by Western blotting. (D) Caspase-1 activity in RAW264.7 cells. (E) Detection of IL-1β expression in the SN by ELISA. *P<0.05 vs the control group, and #P<0.05 in comparison with the respective unprimed macrophages. (F–I) The pro-caspase-1, pro-IL-1β, and processed caspase-1 and IL-1β expression levels, (J) caspase-1 activity, and (K) IL-1β release in mouse macrophages stimulated (or not) with NiONPs in the presence (or not) of z-yvad-fmk. Data are presented as mean ± SEM from three independent experiments. *P<0.05, **P<0.01 vs the control group, and #P<0.05 in comparison with the respective unprimed macrophages.

Abbreviations: ELISA, enzyme-linked immunosorbent assay; IL, interleukin; LPS, lipopolysaccharides; NiONP, nickel oxide nanoparticle; SEM, standard error of the mean; SNs, supernatants.