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| Reagent | Final concentration |
|---|---|
| DMEM/F12 | 1× |
| Fetal bovine serum (FBS) | 5% |
| Gentamicin | 50 μg/mL |
| Insulin | 5 μg/mL |
| Trypsin | 2 mg/mL |
| Collagenase | 2 mg/mL |
Combine reagents and shake at 37°C until collagenase is dissolved (~15 min). Filter sterilize through a 0.2-μm filter. Store at 4°C for up to 1 mo.
