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. Author manuscript; available in PMC: 2016 Aug 18.
Published in final edited form as: Anal Chem. 2015 Jul 31;87(16):8399–8406. doi: 10.1021/acs.analchem.5b01745

Figure 1. Schematic representation of the integrated top-down LC/MS+-based method for quantification of α-actin isoforms.

Figure 1

Tissues are homogenized in HEPES buffer; the myofilament proteins are extracted by 1% TFA buffer and separated using LC/MS. The α-actin fraction is then collected and analyzed using a high-resolution mass spectrometer.