Skip to main content
. Author manuscript; available in PMC: 2016 Aug 18.
Published in final edited form as: Anal Chem. 2015 Jul 31;87(16):8399–8406. doi: 10.1021/acs.analchem.5b01745

Figure 3. Comprehensive characterization of α-cardiac actin (αCAA) by top-down high-resolution MS.

Figure 3

(A) The fragmentation map of αCAA based on ECD and CAD spectra, Ac-represents acetylation, and the green circle represents methylation. (B) The representative b and y ions from CAD experiments. Fragmentation assignments were made based on the sequence of human αCAA (P68032, ACTC_HUMAN, UniProtKB/Swiss-Prot) with acetylation at the N-terminus and methylation at His73. Circles represent the theoretical isotopic abundance distribution of the isotopomer peaks corresponding to the assigned mass. Calc'd, calculated molecular weight; Expt'l, experimental molecular weight.