Examples of depolarization induced by picospritzed HA and the effect of ERα agonist, PPT, and the lack of an effect of ERβ agonist, DPN. A, PPT potentiated the depolarization induced by HA. B, DPN had no effect on a separate neuron. Each series of traces are segments of a continuous recording from a neuron with gaps denoted by dashed lines. Arrows below the traces indicate the time points when HA were picospritzed. The number (in mV) near the peak of responses indicates the extent of depolarization induced by HA. PPT and DPN were applied through the bath.