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. Author manuscript; available in PMC: 2017 Aug 1.
Published in final edited form as: Arterioscler Thromb Vasc Biol. 2016 Jun 16;36(8):1627–1637. doi: 10.1161/ATVBAHA.116.307812

Figure 2. Cad-11 overexpression leads to formation of calcific lesions and aortic stenosis.

Figure 2

A–C, Von Kossa staining reveals greater mineralization (arrows, B) in adult Cad11 OX mice compared to WT mice at 10 months (n≥9, **p<8E-9) Scale bar=200μm. D–F, Alizarin Red staining reveals greater calcification (arrows, E) in adult Cad11 OX mice compared to WT mice at 10 months (n=4, *p<0.0005) G–I, Blood ejection velocity through the AoV was evaluated using Doppler ultrasound at 10 months. Arrows in H indicate regurgitation while arrowheads indicate elevated outflow velocity compared to WT mice (n=10, *p<0.0005). Significance was determined using the Student’s t-test at p<0.05.