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. Author manuscript; available in PMC: 2017 Aug 1.
Published in final edited form as: Arterioscler Thromb Vasc Biol. 2016 Jun 16;36(8):1627–1637. doi: 10.1161/ATVBAHA.116.307812

Figure 3. GTP-RhoA and Sox9 are upregulated in Cad11 OX AoVs.

Figure 3

A–C, Active GTP-bound RhoA (GTP-RhoA) expression is higher in Cad11 OX mice at 10 months throughout the aortic valve. Normal GTP-RhoA expression in WT mice is mostly restricted to the valve endothelium (white arrows) whereas expression in Cad11 OX is high in both the endothelium and interstitium (white arrowheads) (n≥5, *p<0.01). D–G, Nuclear and overall Sox9 expression are higher in Cad11 OX mice at 10 months as measured by whole valve fluorescence and colocalization with DRAQ5-stained nuclei. Significance was determined using the Student’s t-test (n≥5, **p<0.005, ***p<1E-8) Scale bar=100μm.