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. Author manuscript; available in PMC: 2017 Aug 1.
Published in final edited form as: Arterioscler Thromb Vasc Biol. 2016 Jun 16;36(8):1627–1637. doi: 10.1161/ATVBAHA.116.307812

Figure 5. Cad-11 overexpression increases stress fiber bundles and protrusions.

Figure 5

Control PAVICs seeded at a low density (A,D) contain only lamellipodia while Cad-11 overexpressing (Cad11) PAVICs (B,E) exhibit stress fibers and multiple filopodia (white arrowheads) two days post-transfection. Cad-11 overexpressing PAVICs treated with Y27632 exhibit no stress fiber bundles, but have multiple filopodia (white arrowheads, C,F) (Scale bar=50μm for A–C, Scale bar=25μm for D–F). Quantification of the ratio of filopodia to lamellipodia (G). Bars that do not share any letters are significantly different according to a one-way ANOVA with Tukey’s post-hoc test (p<0.01, n≥12 cells). Control PAVICs seeded at a high density have fewer stress fiber bundle positive cells (H, white arrows) compared to Cad-11 transfected cells (I). Cad-11 overexpressing cells treated with Y27632 have few stress fiber bundles (J). Quantification of percent stress fiber bundle positive cells (K). Bars that do not share any letters are significantly different according to a one-way ANOVA with Tukey’s post-hoc test (p<0.01, n≥13 groups of at least 4 cells, Scale bar=50μm for H–J).