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. Author manuscript; available in PMC: 2017 Aug 1.
Published in final edited form as: Arterioscler Thromb Vasc Biol. 2016 Jun 16;36(8):1627–1637. doi: 10.1161/ATVBAHA.116.307812

Figure 6. Cad-11 overexpression increases calcification but is attenuated with ROCK inhibition.

Figure 6

A–C, Alizarin Red staining shows calcific nodules of PAVICs placed in osteogenic growth media (OGM) after 10 days. D,E, Quantification of calcific nodules per well and Alizarin Red activity. Scale bar=0.5mm. F, qRT-PCR shows differences in gene expression among PAVICs in the three conditions compared to OGM. Bars that do not share any letters are significantly different according to a one-way ANOVA with Tukey’s post-hoc test (p<0.01, n=4).