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. Author manuscript; available in PMC: 2017 Oct 1.
Published in final edited form as: Helicobacter. 2016 Jan 28;21(5):395–404. doi: 10.1111/hel.12300

Figure 1.

Figure 1

H. pylori associates with mouse gastric GSM06 epithelial cells and translocates CagA. (A) Epithelial cells grown on glass coverslips were infected with HpM5, washed, fixed, and stained with H. pylori-specific mouse antisera and anti-mouse IgG-FITC conjugate. Cellular morphology was detected using phalloidin and DAPI counter-stains. (B) Western blot analysis of bacterial lysate from strain 26695, 26695 with a PAI deletion (26695 CagA), mouse-adapted HpM5 and the isogenic mutant HpM5 aph::cagA (HpM5 CagA) using anti-CagA polyclonal antibody. (C) CagA-specific immunoblot analysis of AGS and GSM06 cell lysates following infection with HpM5 demonstrating phospho-CagA (P-CagA) and total CagA (T-CagA).