Table 1.
Virulence attributes and anatomic source of C. tropicalis.
| Isolates | PrZ_48h | HZ_72h | PMP | TCC-SUP | PZ_72h | Filamentation ability | Percentage of cell damage | Anatomic source | ||
|---|---|---|---|---|---|---|---|---|---|---|
| LA | BF_CV | Ad_XTT | BF_XTT | |||||||
| CYCT01 | 0.504 (M) | 0.474 (M) | 0.014 (M) | 0.001 (L) | 0.019 (M) | 0.018 (L) | 0.564 (M) | M | 0.1448 (M) | feces |
| FXCT01 | 0.443 (M) | 0.461 (M) | 0.003 (L) | 0.052 (L) | 0.046 (M) | 0.187 (M) | 0.567 (M) | L | 0.3404 (H) | blood |
| ZRCT08 | 0.452 (M) | 0.535 (M) | 0.033 (H) | 0.187 (M) | 0.112 (H) | 0.177 (M) | 0.512 (M) | L | 0.2178 (M) | sputum |
| ZRCT28 | 0.394 (H) | 0.496 (M) | 0.005 (M) | 0.001 (L) | 0.065 (H) | 0.031 (L) | 0.490 (M) | H | 0.1745 (M) | urea |
| ZRCT32 | 0.465 (M) | 0.442 (M) | 0.018 (H) | 2.954 (H) | 0.024 (M) | 0.200 (M) | 0.474 (M) | H | 0.0328 (L) | feces |
| ZRCT45 | 0.596 (M) | 0.515 (M) | 0.019 (H) | 1.576 (H) | 0.186 (H) | 0.248 (H) | 0.512 (M) | N | 0.2197 (M) | sputum |
| ZRCT52 | 0.461 (M) | 0.444 (M) | 0.009 (M) | 0.023 (L) | 0.006 (L) | 0.171 (M) | 0.505 (M) | H | 0.0234 (L) | sputum |
| ZRCT63 | 0.585 (M) | 0.518 (M) | 0.014 (M) | 1.404 (H) | 0.041 (M) | 0.552 (H) | 0.519 (M) | L | 0.0543 (L) | sputum |
| ZRCT64 | 0.626 (L) | 0.514 (M) | 0.003 (L) | 0.351 (M) | 0.012 (L) | 0.045 (L) | 0.657 (L) | M | 0.0604 (L) | sputum |
| ATCC750 | 0.413 (M) | 0.557 (M) | 0.033 (H) | 0.171 (M) | 0.022 (M) | 0.024 (L) | 0.508 (M) | M | 0.1939 (M) | bronchus |
“H” indicates high activity; “M” indicates medium activity; and “L” indicates low activity.
PrZ_48 h = colony diameter/total diameter of the colony plus precipitation halo; which indicates the activity of secreted aspartyl proteases.
HZ_72 h = colony diameter/total diameter of the colony plus hemolysis halo; which indicates the activity of hemolysins.
PZ_72 h = colony diameter/total diameter of the colony plus precipitation halo; which indicates the activity of esterases.
PMP, polystyrene flat-bottomed microtitre plates; TCC-SUP, human urinary bladder epithelial cell line. CV, crystal violet; XTT, 2, 3-bis (2-methoxy-4-nitro-5-sulfo-phenyl)-2H-tetrazolium-5-carboxanilide; LA, late adhesion on PMP surface; BF_CV, BF on PMP surface determined by CV assay; Ad_XTT, adhesion on TCC-SUP surface quentified by XTT assay; BF_XTT, BF on TCC-SUP surface quantified by XTT assay.
The cut-offs for classification are as follows: PrZ_48 h, HZ_72 h, and PZ_72 h (H < 0.41, 0.41 ≤ M < 0.61, 0.61 ≤ L < 1.00); LA (L < 0.004, 0.004 ≤ M < 0.015, 0.015 ≤ H); BF_CV (L < 0.075, 0.075 ≤ M < 0.881, 0.881 ≤ H); Ad_XTT (L < 0.016, 0.016 ≤ M < 0.052, 0.052 ≤ H); and BF_XTT (L < 0.062, 0.062 ≤ M < 0.204, 0.204 ≤ H).