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. 2016 Jul 29;7:284. doi: 10.3389/fimmu.2016.00284

Figure 2.

Figure 2

Epicutaneous CpG ODN induces long-term CTL memory. C57BL/6 mice immunized with scOVA ± ecCpG or scCpG ODN were restimulated with scOVA antigen at the same site of immunization 100 days post-immunization. Lungs and spleen were collected for flow cytometric analysis and OVA-specific CD8 T cells were enumerated. (A) Representative flow cytometric plots of the B220CD8+ population, gating for OVA tetramer and CD8 double-positive cells. (B) The percentage and (C) total number of OVA-specific tetramer+ cells among CD8+ T cells in the lungs and spleen. Bar graphs show the mean ± SD from two independent experiments (n = 4–6). Statistical significance of all four groups was analyzed by one-way ANOVA test with a Tukey’s multiple comparison post hoc analysis *p < 0.05.