Figure 6.

Effect of amantadine on DEER data is almost negligible for WT M2TMD₂₁₋₄₉ residing in DLPC/DLPS membranes. Background subtracted and normalized DEER time-domain data (left) and corresponding distances (right) for spin-labeled residue L46C at P/Ls of 1:160, 1:500, and 1:1300 are shown. Only the early 1.1 μs of the DEER signals are shown for clarity. No detectable or very small changes in the DEER signals and corresponding distances were observed upon addition of amantadine. Longer incubation with amantadine (1 h at RT) did not produce any additional effect on the DEER data (gray signal in the middle left panel for P/L of 1:500) compared to standard incubation of ~4 min.