Table 4.

Aspergillus and azole resistance profile in matched pairs of BA and BAL samples.

Patient	Specimen	Underlying disease	Clinical diagnosis	Culture/azole susceptibility	Pan-Aspergillus PCR	Ct	ΔCt (BAL−BA)*	CYP51A PCR	Mutations
A	BA	CF+SOT	Colonization	Negative	Pos.	31.8		Pos.	None
	BAL	CF+SOT	Colonization	Negative	Neg.	No Ct	8.2	Neg.	NA
B	BA	SOT	Colonization	Negative	Pos.	31.7		Pos.	None
	BAL	SOT	Colonization	Negative	Pos.	33.4	1.7	Pos.	I217T
C	BA	HAEM	IA	Negative	Pos.	28.4		Pos.	None
	BAL	HAEM	IA	Negative	Pos.	35.9	7.5	Neg.	NA
D	BA	HAEM	Colonization	Negative	Pos.	33.4		Pos.	L399F
	BAL	HAEM	Colonization	Negative	Neg.	36.2	2.8	Neg.	NA
E	BA	CF+SOT	Colonization	Penicillium spp.	Pos.	30.6		Pos.	None
	BAL	CF+SOT	Colonization	Negative	Pos.	34.8	4.2	Neg.	NA
F	BA	SOT	Colonization	Negative	Pos.	33.9		Pos.	None
	BAL	SOT	colonization	Negative	Neg.	36.2	2.3	Neg.	NA
G	BA	SOT	IA	A. fumigatus/susceptible	Pos.	24.9		Pos.	None
	BAL	SOT	IA	A. fumigatus/susceptible	Pos.	27.1	2.3	Pos.	None
H	BA	HAEM	IA	A. fumigatus/susceptible	Pos.	29.6		Pos.	None
	BAL	HAEM	IA	A. fumigatus/susceptible	Pos.	34.4	4.9	Pos.	None
I	BA	PULM	ABPA	Negative	Pos.	31.4		Pos.	R65R
	BAL	PULM	ABPA	Negative	Neg.	No Ct	8.7	Neg.	NA
J	BA	HAEM	IA	A. fumigatus/susceptible	Pos.	32.4		Pos.	None
	BAL	HAEM	IA	Negative	Pos.	31.8	−0.6	Pos.	None
K	BA	HAEM	IA	Negative	Neg.	No Ct		Neg.	NA
	BAL	HAEM	IA	Negative	Pos.	34.1	−5.9	Pos.	None

NA, not applicable.

^*A Ct-value of 40 was used to calculate Ct difference between BA and BAL samples when no Ct was reported for pan-Aspergillus PCR.