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. 2016 Jul 29;6:80. doi: 10.3389/fcimb.2016.00080

Figure 5.

Figure 5

R. montanensis is rapidly destroyed in THP-1-derived macrophages. THP-1-derived macrophages were infected with R. montanensis (MOI = 10). At 60 min or 24 h post infection, cells were fixed, permeabilized, and double stained for immunofluorescence confocal microscopy analysis with NIH/RML I7198 followed by Alexa Fluor 546 (red) to stain R. montanensis, and the monoclonal antibody for LAMP-2, lysosomal membrane protein followed by Alexa Fluor 488 (green). (A–D) Representative images of a single slice from the z stacks. THP1-derived macrophages at 60 min post infection (A,B) and 24 h post infection (C,D). Each row shows, from left to right, Rickettsia staining, LAMP-2 staining, the merged image, and a RGB plot profile illustrating the fluorescence intensity along the magenta arrow. Scale bar = 10 μm. Supplementary Movies 1, 2 represent 360° rotation movie of the 3D projection of the stack images shown in (A) and (C), respectively.