Fig. 1.

Methylation analysis of 58 patients with childhood-onset non-autoimmune diabetes. A: Genomic structure of PLAGL1 and its flanking CpG sites. C1–7 represent cytosines at the CpG sites in the differentially methylated region. Forward (F) and reverse (R) primers were used for PCR amplification and the Seq primer was used for pyrosequencing. B: Results of the methylation analysis. Black dots represent the methylation statuses of the 58 patients. Gray shaded areas indicate the reference range obtained from 49 control individuals. The red dots depict the results of three previously reported patients with 6q24 uniparental disomy (5).