Fig. 2.

Identification of the sequence variation of FGFR3. A: Partial sequence of the PCR product, and schematic diagrams of the FGFR3 protein. The chromatogram represents a heterozygosity of histidine (CAC) in place of leucine (CTC) at codon 324, which is located at the third immunoglobulin-like domain in the extracellular region of FGFR3. The arrow indicates the mutated nucleotide. B: Leu324 is a highly evolutionarily conserved amino acid among several other species, not only in FGFR3 but also in the other three FGFRs.