. 2016 Mar 31;35(3):113–121. doi: 10.12938/bmfh.2015-023

Table 1. Sequence of primers used in this study.

Exp.	Aim		Primer set	Thermal cycling program
1	Const. pColdII-15730	eqlA Fw	CATCATCATCATCATATGAAGAACAAGTTCTATCC	[98ºC 10 sec – 58ºC 5 sec – 72ºC 10 sec] × 30 cycle
1	amplify EGYY15730 gene	eqlA Rv	GCTTGAATTCGGATCCCTACAGGTTGCAGCCAGCG	[98ºC 10 sec – 58ºC 5 sec – 72ºC 10 sec] × 30 cycle

2	Const. pColdII-15750	eqlB Fw	CATCATCATCATCATATGGCACAGGAAGTC	[98ºC 10 sec – 60ºC 5 sec – 72ºC 15 sec ] × 30 cycle
2	amplify EGYY 15750 gene	eqlB Rv	GCTTGAATTCGGATCCTTAGACCTCGATCTCGCCCTG	[98ºC 10 sec – 60ºC 5 sec – 72ºC 15 sec ] × 30 cycle

3	Const. pColdII-15760	eqlC Fw	CATCATCATCATCATATGGCACAATTCGATGTTGAG	[98ºC 10 sec – 62ºC 5 sec – 72ºC 10 sec ] × 30 cycle
3	amplify EGYY15760 gene	eqlC Rv	GCTTGAATTCGGATCCCTACATAGTGGAGATCGCGTGG	[98ºC 10 sec – 62ºC 5 sec – 72ºC 10 sec ] × 30 cycle

4	Confirm eqlA of strain	seq_eqlA_Nde	GTCCATGAACACGATGC	98ºC 5 min [98ºC 0 sec – 50ºC 5 sec – 60ºC 4 min ] × 25 cycle
4	YY7918 in pColdII	seq_eqlA_Bam	TCGACCAGACAATCATC

5	Confirm eqlB of strain	seq_eqlB_Nde	ATCATCACCTCCTCCACC	[98ºC 10 sec – 62ºC 5 sec – 72ºC 10 sec ] × 30 cycle
5	YY7918 in pColdII	seq_eqlB_Bam	TAGTCAGACAGGTCGAC	[98ºC 10 sec – 62ºC 5 sec – 72ºC 10 sec ] × 30 cycle

6	Confirm eqlC of strain	seq_eqlC_Nde	AGCTCGTTCTGAATAGAG	98ºC 5 min [98ºC 0 sec – 50ºC 5 sec – 60ºC 4 min ] × 25 cycle
6	YY7918 in pColdII	seq_eqlC_Bam	ACGACGACGCCTTTATGA

Underlining indicates the homologous 15 bp.