TABLE 1.
The primers utilized in this study
| Gene | Description | Sequence |
|---|---|---|
| Real-time RT-PCR | ||
| IFN-β | Sense | 5′-GGCCATGACCAACAAGTGTCTCCTCC-3′ |
| Anti-sense | 5′-ACAGGTTACCTCCGAAACTGAGCGC-3′ | |
| GAPDH | Sense | 5′-CCACTCCTCCACCTTTGAC-3′ |
| Anti-sense | 5′-ACCCTGTTGCTGTAGCCA-3′ | |
| ISG15 | Sense | 5′-CATGGGCTGGGACCTGACG-3′ |
| Anti-sense | 5′-CGCCAATCTTCTGGGTGATCTG-3′ | |
| HBV-RNA | HBV-D/MS-F (64-) | 5′-TGGCTCCAGTTCAGGAACAG-3′ |
| Pre-S2 | HBV-D/S-R (-357) | 5′-GTTGGAGGACAGGAGGTTGG-3′ |
| HBV-RNA | HBV-D/X-F (1386-) | 5′-CTGTGCTGCCAACTGGATCC-3′ |
| X | HBV-D/X-R (-1625) | 5′-GTTCACGGTGGTCTCCATGC-3′ |
| RT-PCR | ||
| IFNAR1 | Sense | 5′-AGTGTTATGTGGGCTTTGGATGGTTTAAGC-3′ |
| Anti-sense | 5′-TCTGGCTTTCACACAATATACAGTCAGTGG-3′ | |
| IFNAR2 | Sense | 5′-ATTTCCATCTATTGTTGAGG-3′ |
| Anti-sense | 5′-CACTTTCTTCTTTCTGTTGA-3′ | |
| SNC#2-NS5A | Sense | 5′-GGACTTGCTGGAAGACACTG-3′ |
| Anti-sense | 5′-GGCATTCACCAGGAACTCGA-3′ | |
| GAPDH | Sense | 5′-CATGTTCGTCATGGGTGTGAACCA-3′ |
| Anti-sense | 5′-AGTGATGGCATGGACTGTGGTCAT-3′ | |
| Real time PCR | ||
| HBV-DNA | Sense (2983-) | 5′-AAGGTAGGAGCTGGAGCATTCG-3′ |
| Anti-sense (-3093) | 5′-AGGCGGATTTGCTGGCAAAG-3′ | |
| Probe | 5′FAM-AGCCCTCAGGCTCAGGGCATAC-3′TAMRA | |