FIGURE 1.

miR-30 is predicted to target the 3′-UTR of SOX9 and is differentially expressed across functionally distinct cell types of the intestinal epithelium. a, cartoon depicting the SOX9 3′-UTR. miRNAs with predicted target sites conserved between mouse and human are labeled. Below, we show the conservation of the predicted miR-30 target site (red text) across various species (TargetScan6.2). miR-30b and miR-30e targeting are shown in detail with predicted base paring colored in red. b, RT-PCR of Sox9 and miRNAs with predicted target sites in the Sox9 3′-UTR across functionally distinct mouse jejunal intestinal epithelial cells (n = 2–4). Significance was determined by Student's two-tailed paired t test relative to non-sorted intestinal epithelial cells. ♭, p < 0.001; ♮, p < 0.01; #, p < 0.05.