FIGURE 5.

miR-30 target genes in intestinal epithelial cells are over-represented in the ubiquitin ligase pathway. a and b, Gene Ontology Molecular Function enrichment analysis using Enrichr identifies three terms as enriched in the up-regulated gene lists across time points. p value (a) and combined scores (b) shown. c, relative SOX9 protein expression in Caco-2 cells subject to mock transfection (n = 2 each) or 100 nm LNA30bcd transfection (n = 3 each) at 72 h post-transfection following 4 h of treatment with either 25 μm MG132 or vehicle (DMSO). The bottom panel shows a representative Western blot. d, cartoon showing model of miR-30 regulation of SOX9 mRNA and protein expression levels.