FIGURE 6.

miR-30 promotes proliferation and inhibits enterocyte differentiation. a, RQV of mean CPM of markers of proliferation in 100 nm LNA30bcd-treated HIECs across time points. Significance was determined by using edgeR generalized linear model accounting for treatment and time. ♭, FDR < 0.001; ♮, FDR < 0.01; #, FDR < 0.05. b, RQV of [³H]thymidine incorporation in HIECs subjected to mock transfection or LNA30bcd transfection (10 or 100 nm) at 48 h post-transfection (n = 12 each). Significance was determined by two-tailed unpaired Student's t test. ♭, p < 0.001; ♮, p < 0.01; #, p < 0.05. c, RQV of mean CPM of HES1 (Hairy and Enhancer of Split 1) in 100 nm LNA30bcd-treated HIECs across time points (n = 3 each). Significance was determined by generalized linear model accounting for treatment and time. ♭, FDR < 0.001; ♮, FDR < 0.01; #, FDR < 0.05. d, RQV of miR-30c by RT-PCR in Caco-2 cells at 72 h and 21 days after either mock transfection (72 h, n = 9; 21 days, n = 6), 100 nm LNA30bcd transfection (72 h, n = 9; 21 days n = 6), 100 nm LNA101* (72 h, n = 6; 21 days, n = 3), or 100 nm LNA320a (72 h, n = 9; 21 days, n = 6). Significance was determined using two-tailed unpaired Student's t test. ♭, p < 0.001; ♮, p < 0.01; #, p < 0.05. e, RQV of sucrase isomaltase (SI) by RT-PCR in in Caco-2 cells at 72 h and 21 days after either mock transfection (72 h, n = 9; 21 days n = 8), 100 nm LNA30bcd (72 h, n = 9; 21 days, n = 9), 100 nm LNA101* (72 h, n = 6; 21 days, n = 6), or 100 nm LNA320a (72 h, n = 9; 21 days, n = 9). Significance was determined using two-tailed unpaired Student's t test. ♭, p < 0.001; ♮, p < 0.01; #, p < 0.05.