TABLE 2.
Microbial strains used in study
| Strain | Characteristics | Source |
|---|---|---|
| S. agalactiae | ||
| NEM316 | Wild-type (serotype III) | Laboratory stock |
| L. lactis | ||
| NZ9800 | Wild type | Laboratory stock |
| UB2658 | pMSP.bspA+ | This study |
| UB2635 | Empty pMSP | This study |
| C. albicans | ||
| SC5314 | Wild-type | Gillum et al. (70) |
| E. coli | ||
| K12 MG1655 | F− λ− rph-1 | Laboratory stock |
| StellarTM | F′ endA1 supE44 thi-1 recA1 relA1 gyrA96 phoA Φ80d lacZΔ M15 Δ (lacZYA-argF) U169 Δ (mrr-hsdRMS-mcrBC) ΔmcrA, λ− | Clontech |
| B834 (DE3) | F− ompT hsdSB (rB− mB−) gal dcm met (DE3) | Novagen |
| BL21 (DE3) | F− ompT hsdSB (rB− mB−) gal dcm (DE3) | Novagen |
| UB2878 | pOPINF.bspA+; full-length; BL21 | This study |
| UB2879 | pOPINF.bspA-V+; BL21 | This study |
| UB2880 | pOPINF.bspA-C+; 2231G>A; BL21 | This study |
| UB2881 | pOPINF.bspA-C+; BL21 | This study |
| UB2882 | pOPINF.bspA-A+; BL21 | This study |
| UB2883 | pOPINF.bspA-AΔAsn+; BL21 | This study |
| UB2884 | pOPINF.bspA-P+; BL21 | This study |