TABLE 1.

Bacterial strains and plasmids used in this study

Strains or plasmids	Relevant characteristicsa	Reference or source
Bacterial strains
V. vulnificus
MO6-24/O	Clinical isolate; virulent	Laboratory collection
OH0703	MO6-24/O with oxyR2::nptI; Kmr	Ref. 11
E. coli
SM10 λ pir	thi thr leu tonA lacY supE recA::RP4–2-Tc::Mu λpir; Kmr; host for -requiring plasmids; conjugal donor	Ref. 23
BL21 (DE3)	F− ompT hsdS (rB−mB−) gal (DE3)	Laboratory collection
Plasmids
pJH0311	0.3-kb MCS of pUC19 cloned into pCOS5; Apr, Cmr	Ref. 26
pDY1025	pJH0311 with wild-type oxyR2; Apr, Cmr	Ref. 11
pBANG1416	pJH0311 with the mutant oxyR2 encoding OxyR2-C206D; Apr, Cmr	This study
pET-28a(+)	His6-tag fusion protein expression vector; Kmr	Novagen
pDY1001	pET-28a (+) with wild-type oxyR2; Kmr	Ref. 11
pDY1104	pET-28a (+) with the mutant oxyR2 encoding OxyR2-C206S; Kmr	This study
pDY1014	pET-28a (+) with the mutant oxyR2 encoding OxyR25CA; Kmr	Ref. 11
pBANG1501	pET-28a (+) with the mutant oxyR2 encoding OxyR25CA-C206D; Kmr	This study

^a Ap^r, ampicillin-resistant; Cm^r, chloramphenicol-resistant; Km^r, kanamycin-resistant.