TABLE 2.
Oligonucleotides used in this study
| Name | Oligonucleotide Sequence (5′→3′)a,b | Use |
|---|---|---|
| For mutagenesis | ||
| OXYR2C206D-F | GGAAAAAGAGCATGATCTGACTGAACACGCGGTGTCGG | Construction of OxyR2-C206D mutant |
| OXYR2C206D-R | CCGACACCGCGTGTTCAGTCAGATCATGCTCTTTTTCC | |
| OXYR2C206S-F | GGAAAAAGAGCATAGTCTGACTGAACAC GCGGTGTCGGCC | Construction of OxyR2-C206S mutant |
| OXYR2C206S-R | GGCCGACACCGCGTGTTCAGTCAGACTATGCTCTTTTTCC | |
| For quantitative real-time PCR | ||
| PRX2QRT-F | GTTGCTTTCCGTGGCTCTTTCC | Expression of prx2 |
| PRX2QRT-R | TACTTCGCCGTGCTTCTGGTG | |
| OXYR2QRT2-F | GCCCAGTCTGAAGCAGTTACACTATC | Expression of oxyR2 |
| OXYR2QRT2-R | GAGAGGACAGCCGATCAACTCTTC | |
| PRX1QRT-F | TACCCAGCGGACTTCACTTTCG | Expression of prx1 |
| PRX1QRT-R | TGACACTGAGTACACTTCCACACC | |
| KATGQRT-F | CGTTCCGTTTGCAGCAGGTC | Expression of katG |
| KATGQRT-R | CCAGTTGCGGAAGCCATCTG | |
| For DNA footprinting | ||
| PRX2P02-F | GCAGAGTAATAAGATAGTGTAACTGC | Amplification of prx2 upstream region |
| PRX2P02-R | CCTACTAGTACCATTTTTTTGCTCC | |