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Assessing interactions between lobes. Comparison of the spectra of the isolated apo (A and B) and holo (C and D) forms of the N and C lobes with full-length F2TnC. In both images, full-length F2TnC is shown in black and the N lobe and the C lobe in red. The HSQCs of the holo fragments almost completely overlap on the full-length construct, indicating the absence of interaction between lobes. This is not completely true for the apo forms.
