FIGURE 1.

PA2799 (or HapZ) interacts with the phosphoreceiver domain of SagS as demonstrated by bacterial two-hybrid assay. A, domain organization of the hybrid histidine kinase SagS (DHp: dimerization and histidine phosphotransfer domain; CA: catalytic and ATP-binding domain; REC: phosphoreceiver domain). The fragments found to interact with PA2799 are represented by bars. B, colonies from bacterial two-hybrid assay indicate the interaction between PA2799 and various SagS constructs (ΔN317 denotes the construct that lacks the N-terminal 317 residues; − denotes empty pBT vector; + denotes pBT-HapZ vector). C, specific bacterial two-hybrid assays demonstrate the specific interaction between PA2799 (or HapZ) and SagS.