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Stern-Volmer plots of holoBcII tryptophan fluorescence, in the presence of 0.1 mm ZnSO4, quenched by acrylamide at several GdmCl concentrations. Experiments were performed for the native state in the absence of GdmCl (□), the intermediate state in the presence of 2.4 m GdmCl (○), and the unfolded state in the presence of 3.5 m GdmCl (▿). Data are the average of at least three independent experiments, and error bars represent standard deviations.
