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. 2016 May 23;291(31):16186–16196. doi: 10.1074/jbc.M116.718551

FIGURE 5.

FIGURE 5.

Implications of the secretion of various sgp130 forms for cell proliferation. A and B, equal amounts of Ba/F3-gp130-GFP, Ba/F3-gp130-sgp130(Fc), Ba/F3-gp130-sgp130-RAPS(Fc), and Ba/F3-gp130-sgp130-E10(Fc) cells were incubated for 24 h. Supernatants were harvested, and the amount of sgp130 in the cell culture supernatant was quantified via ELISA (mean ± S.D., n = 3). C and D, Ba/F3-gp130 cells stably transduced with various forms of sgp130 were stimulated with increasing amounts of hyper-IL-6 (0.001–100 ng/ml), and cell proliferation was measured after 48 h. Data shown are the mean ± S.D. (n = 3 biological replicates) from one representative experiment of three performed with similar outcomes. RLU, relative light unit. E, Ba/F3-gp130 cells were incubated with either 1, 10, or 100 ng/ml hyper-IL-6 and increasing amounts (0–1000 ng/ml) of recombinant sgp130Fc, and cell proliferation was determined after 48 h. F, Ba/F3-gp130 cells stably transduced with sgp130, sgp130-RAPS, or sgp130-E10 as well as Ba/F3-gp130 cells (wild-type) were grown for 48 h, and their supernatants were used for 48-h cultivation of Ba/F3-gp130 cells in the presence of various amounts of hyper-IL-6. Cell proliferation was measured after this 48-h incubation with the supernatants. Data are the mean ± S.D. of three independent experiments (n = 3 biological replicates each).