FIGURE 7.

Tat K71me regulates Tat transactivation. A, TZMBL cells were transfected with increasing concentrations of plasmids (2, 5, and 10 ng) expressing WT or mutant Tat101. Luciferase values are shown from four independent experiments measured as fold expression over empty vector (mean ± S.E.). Western blot analysis was performed in cells transfected with 100 ng of Tat-FLAG plasmid and blotted with anti-FLAG antibodies. B, luciferase values in TZMBL cells transduced with KMT7b or scrambled shRNAs and then transfected with WT or TatK71R Tat-expressing plasmids (20 ng). KMT7 knockdown is visualized by Western blotting. Values are mean ± S.E. from four independent experiments. Significance was calculated with a one-sided t test. *, p < 0.05.