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. 2016 May 23;291(31):16249–16262. doi: 10.1074/jbc.M115.709675

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© 2016 by The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 5. — RET^ΔE3 and RET^ΔE345 can heterodimerize with full-length RET. A, HA epitope tagged RET51 (RET51::HA) was co-transfected with untagged RET51^Δ^E3 or RET51^Δ^E345 in HEK293T cells. Immunoprecipitations (IP) were performed with an HA antibody to select for RET51::HA. Immunoblotting (IB) for RET51 showed co-immunoprecipitation of RET51^ΔE3 and RET51^ΔE345 with full-length RET51. B, a similar experiment was performed using FLAG epitope tagged RET51^Δ^E345 (FLAG:: RET51^Δ^E345). Immunoprecipitations with an anti-FLAG antibody selected for FLAG:: RET51^ΔE345. Immunoblotting for RET51 demonstrated the association of RET51 and RET51^ΔE3 with FLAG:: RET51^ΔE345. Co-transfections with TrkA were performed for both experiments as a negative control, and immunoblotting for TrkA showed no binding with either RET51::HA or FLAG::RET51^ΔE345, as expected.