FIGURE 2.

Effects of palm and olive oils on insulin signaling in the mouse intestine. Fasted mice were force-fed water, olive oil, or palm oil, and 4 h later were either subjected or not to an intraperitoneal insulin injection. Mice were euthanized 30 min after insulin injection, and cell lysates from intestinal epithelial cells were analyzed by Western blotting for AKT phosphorylation (P-AKT) at serine 473 (top panel). AKT phosphorylation was reported as the ratio of P-AKT to total AKT content (arbitrary units, a.u.) (bottom panel). The P-AKT/AKT ratio obtained in the water condition without insulin was set at 1. Results display mean ± S.E., n = 4, for each condition. **, p < 0.01, and ***, p < 0.001, compared with the same treatment without insulin; ns, not significant.