FIGURE 7.
A Notch transgene, Ngt-FH, mimics an endogenous copy of Notch. A, diagram of the Ngt-FH ECD, showing 36 EGF repeats, three LNR domains, the heterodimerization (HD) domain, and transmembrane (TM) domain. A triple FLAG tag and a double HA tag were inserted after the EGF repeats and before the LNR repeats. EGF repeats containing an O-fucosylation consensus sequence are colored red. B, a Notch haploinsufficient female wing showing thickened veins (arrowhead) and wing margin loss (arrow). These phenotypes could be rescued with one copy of Ngt-FH (C). D–F, phenotypes exhibited by a Notch-null hemizygous male rescued from embryonic lethality to adulthood with one copy of Ngt-FH. Note the normal leg joints (arrows in D), normal bristle pattern on the thorax (E), and normal wings (F). The arrowhead in D marks the sex comb, which is a male-specific feature of the Drosophila legs.