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. 2016 Jul 29;6:30812. doi: 10.1038/srep30812

Figure 1. MT mediated cardiomyocyte apoptosis.

Figure 1

(A) Traumatic injury caused cardiomyocyte apoptosis for 0 r (a,e), 100 r (b,f), 200 r (c,g) and 400 r (d,h) (n = 5). Total nuclei (a–d) were determined using DAPI staining (blue), and apoptotic nuclei (e–h) were identified using positive TUNEL staining (green). (B) The apoptotic index was calculated by counting TUNEL signals in 5 randomly selected crypts after TUNEL staining, as in A. Statistical comparisons were performed using one-way analysis of variance. *P < 0.05, **P < 0.01 vs. sham.